Biochemical

Tilmicosin is a macrolide antibiotic drug, mainly for anti-animal and bacterial infection. Many
countries make its MRLs in food for safety control. Tilmicosin ELISA kits utilizing the latest
biotechnology development has easy, rapid, and sensitive advantages.

This kit uses indirect-competition method mainly based on the immunological reaction
between specific antigen and antibody. Tilmicosin specific antigen coated on microtiter plates
competes binding sites of enzyme-labeled Tilmicosin specific antibody with added calibrator
(or sample). With TMB chromogenic substrate, the absorbance value was negatively correlated
with residues of Tilmicosin. Under the calibration curve, Tilmicosin content of the sample can
be quantified multiplied by the corresponding compare dilution.

Detectable samples
Tilmicosin residue can be quantitative of tissue, honey and milk.

Specificity
Tilmicosin-------------------------------------------------------100%
Tylosin -----------------------------------------------------------<1%
Spectinomycin, Lincomycin --------------------------------0.1%
Detection limit:
0.5ppb is the lowest detection limit of Tilmicosin kit. Linear regression is between 0.5-13.5ppb.

Sensitivity:
Tissue (Method 1)---------------------------------------5ppb
Tissue (Method 2)---------------------------------------1ppb
Honey (Method 1)---------------------------------------5ppb
Milk (Method 2)-----------------------------------------10ppb

Recovery rate:
Tissue (Method 1)----------------------------------60-120%
Tissue (Method 2)--------------------------------- 60-120%
Honey ----------------------------------------------- 60-120%
Milk --------------------------------------------------- 60-120%