Chemical Reagent

Product Description
REAGEN? Sulfonamide Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect sulfonamide antibiotic residues in raw commingled cow’s milk. This rapid test uses a broad-spectrum binding protein which eliminates the need for a heating block, and is designed for rapid field.Examples of sulfonamide antibiotics and their limits of detection using the Sulfonamide Strip Test Kit are shown in Table 1.
The features of the kit are:
? ? Rapid strip test method –7 minutes
? ? Novel binding protein that requires no heating step
? ? High sensitivity
? ? High reproducibility

Mechanism of Reaction
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the sulfonamide binding protein on the gold particles to engage with any sulfonamide antibiotic residues present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to the sulfonamide antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the sulfonamide binding protein on the gold particle has engaged with antibiotic present in the milk