Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme - SRT0202
Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme For Sale, Most Competitive Price, Fast Delivery, Custom Service, Wholesale Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme, Made in China, High Quality Products!, China cheap prodocuts, china suppliers Supplier, Manufacturer.
Sequencing Grade TrypsinTrypsin?SRT Sequencing Grade Enzyme
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Description
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Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere
with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin
is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
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Main Features
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Format | Liquid in 50mM HAc, or fluffy solid. |
Protein concentration | 0.5mg/ml in 50mM HAc |
Purity(RP-HPLC) | ≥99% by HPLC |
Contaminant activity | Recombinant porcine trypsin. No any other?proteases?activities contaminant. |
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Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a?
reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
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Reconstitution
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Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to
-25 °C for up to one month. After first reconstitution the product must be stored in appropriate
aliquots to avoid repeated freezing and thawing.
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Specificity
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Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the
basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.
Shanghai Yaxin Biotechnology Company
Address: No.466 YinDu Road,XuHui, Shanghai, China, Shanghai, Shanghai, China, 20000
Tel: 86-21-54336592
Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise
that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only
a company on researching and producing the recombinant carboxypeptidase B and recombinant
trypsin.
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