Sequencing Grade Carboxypeptidase B For Detecting Antibody Proteomics Grade - SRCPB01
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Sequencing Grade Carboxypeptidase B For Detecting Antibody Proteomics Grade
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Description
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Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
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Application
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Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
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Recommend?Usage
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Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH?7-9.
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Storage Instructions
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Recommend recombinant carboxypeptidase B lyophilized?should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
Shanghai Yaxin Biotechnology Company
Address: No.466 YinDu Road,XuHui, Shanghai, China, Shanghai, Shanghai, China, 20000
Tel: 86-21-54336592
Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise
that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only
a company on researching and producing the recombinant carboxypeptidase B and recombinant
trypsin.
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