Chemical Waste

Rice Grains Derived Human Serum Albumin CAS 70024-90-7 For Animal Free Medium

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Quick detail
# # CAS No.: 70024-90-7
# Other Names: rHSA
# EINECS No.: 274-272-6
# Place of Origin: Hubei, China (Mainland)
# Storage and Handling: Stored at temperatures 2-8℃ for 24 months
# Purity: Greater than 99 % as determined by SDS-PAGE
# Source: Rice Grain (Oryza Sativa)
# Endotoxin: Less than 0.25EU/mg


Physiological and Biochemical Properties of rHSA and pHSA

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Char. | pHSA | rHSA |
Amino Acids Sequence | Same | Same |
N-terminus | DAHKSEV | DAHKSEV |
C-terminus | KLVAASQAALGL | KLVAASQAALGL |
Glycosylation | No | No |
Molecular Mass(MALDI) | 66.554KD | 66.550KD |
PI | 4.8 | 4.8 |
Drug Binding | Similar | Similar |
Thermal Stability | M/P 65℃ | M/P 65℃ |
Lipid Conjugation | Identical | Identical |
Crystal Structure | Identical | Identical |
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Description

Healthgen uses State-of-the-art platform called OryzHiExp, having high capacity to express proteins and peptides in the rice grain. The platform uses rice endosperm cells as a bioreactor to specifically and highly express various recombinant proteins and small peptides. The platform is a rice-specific endosperm cell-specific expression platform that focuses on the expression of animal-free products, safe recombinant proteins and peptides. We use this technique to express recombinant human serum albumin to achieve higher safety and biosafety.



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Advantages

1. The chairman of Healthgen Professor Daichang.Yang is one of China& acute;s chief scientists. Professor Yang has rechieved the “Thousand Talents program” of the National Central Organization Department, & quot;Hundred Talents Program& quot; of the Organization Department of Hubei Province and Top 10 & quot;Venture Eagle& quot; entrepreneurs.

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2. With years of effort, Healthgen has developed the State-of-the-Art platform called OryzHiExp, having high capacity to express no animal origin proteins and peptides in the rice endosperm cells.

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3. ?Healthgen Biotechnology Corp. has been committed in research of protein purification and meanwile our technology has been patented by the US Patent and Trademark Office on May 15th 2015. & quot;A method for isolating and purifying recombinant human serum albumin from transgenic rice grain& quot;. The patent number is US9, 023,990. The purity of our rHSA is very high. Now, our rHSA purity can be as high as 99%.

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Supplemental Description?

The research results have been granted with 16 international patents, covering countries, such as United States, Japan, Europe, Canada and South Africa. Parts of the patents are showed as below:

A callus-specific promoter of rice and the use thereof

China patent no. 201110054109.X

Method for expressing small peptides using cereal non-storage proteins as fusion carrier in endosperm and the use thereof

US patent no. US20070289033

Japan patent no. JP2007-325594A

Europe patent no. EP1865064

Canada patent no. CA2587092

China patent no. 200610019285.9

A method for extracting recombinant human serum albumin from transgenic rice grain

South Africa patent no. 2013/04359

China patent no. 201010597544.2

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain;

South Africa patent no. 2013/04358

China patent no. 201010606635.8

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain

South Africa patent no. 2013/04358

US patent no. US9023990 B2

Production of recombinant human serum albumin with rice endosperm cells as biological reactor

China patent no. ZL 2005 1 0019084.4

Based on the innovative technology, Healthgen also published research papers.

Publications

1. He Y, et al. Large-scale production of functional human serum albumin from transgenic rice seeds. Proc Natl Acad Sci USA. 2011;108:19078-19083.

2. Na An, et al. Expression of a Functional Recombinant Human Basic fibroblast Growth Factor from Transgenic Rice Seeds. Int. J. Mol. Sci. 2013, 14, 3556-3567

3. Liping Z, et al. Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed. Journal of Biotechnology. 2012; 164: 300-308.

4. Xie, T, et al. A biologically active rhIGF-1 fusion accumulated in transgenic rice seeds can reduce blood glucose in diabetic mice via oral delivery. Peptides 2008: 29: 1862-1870.

5. Zhen Chen, et al. Human serum albumin from recombinant DNA technology: challenges and strategies. Biochimica et Biophysica Acta. 2013; 1830: 5515-5525.

6. Zhen Chen, et al. Quantitation of the residual DNA from rice-derived recombinant human serum albumin. Analytical Biochemistry. 2014; 450: 4-10.

7. Jiquan Ou, et al. Transgenic rice endosperm as a bioreactor for molecular pharming. Plant Cell Rep (2014) 33:585–594

8. Kai fu, et al. Immunotoxicity assessment of rice-derived recombinant human serum albumin using human peripheral blood mononuclear cells. PLoS ONE 01/2014; 9(8): e104426.