Recombinant enzyme human chymotrypsin proteolytic enzyme CAS 9004-07-3 EC 3.4.21.1 - RCT10
Recombinant enzyme human chymotrypsin proteolytic enzyme CAS 9004-07-3 EC 3.4.21.1 For Sale, Most Competitive Price, Fast Delivery, Custom Service, Wholesale Recombinant enzyme human chymotrypsin proteolytic enzyme CAS 9004-07-3 EC 3.4.21.1, Made in China, High Quality Products!, China cheap prodocuts, china suppliers Supplier, Manufacturer.
Recombinant?Chymotrypsin
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DESCRIPTION
Chymotrypsin?is?a?recombinant?serine?endopeptidase?expressed?in?E.coli,?purified?with?HPLC,?the?gene
?sequence?is?same?as?that?human?chymotrypsin.?The?recombinant?chymotrypsin?hydrolyzes?at?the?carboxyl?
side?of?aromatic?amino?acids:?Tyr,?Phe?and?Trp.?Cleavage?may?also?be?observed,?but?at?a?lower?rate,?at?
Leu?and?Met.?Chymotrypsin?activity?is?optimal?in?the?pH?range?of?7.0–9.0.?This?sequencing?grade?enzyme
?can?be?used?alone?or?in?combination?with?other?proteases?to?produce?protein?digests?for?peptide?mapping?
applications?or?protein?identification?by?peptide?mass?fingerprinting?or?MS/MS?spectral?matching.?It?is?
suitable?for?digestion?reactions?in-solution?or?in-gel.
?
ADVANTAGES
Animal?origin?free:The?use?of?recombinant?chymotrypsin?eliminates?the?content?of?other?proteins,?the
?risk of?virus?presence,?and?other?potential?adventitious?agents?found?in?animal-derived?chymotrypsin.?
Stable:A?sterile?recombinant?chymotrypsin?lyophilized?powder?eliminates?the?contamination?risks?and?
decreases?the?chance?of?activity?loss?in?the?process?of?transport?and?storage.
High?purity:1)Recombinant?Chymotrypsin?provides?increased?specificity?and?eliminates?contaminating?
???????????????activities?found?in?enzymes?purified?from?pancreas.
?????????????2)?No?anion?trypsin?or?cation?trypsin.
?????????????3)?One?single?main?band?at?25kD?by?SDS-PAGE.
?
SPECIFICATION
Serine?endopeptidase?that?specifically?hydrolyzes?peptide?bonds?at?the?C-termini?of?Tyr,?Phe,?and?Trp.?Leu,
?Met,?Ala,?Asp,?and?Glu?are?cleaved?at?a?lower?rate.?Acts?also?upon?amides?and?esters?of?susceptible?amino
?acids.
?
APPLICATION
Hydrolysis?of?proteins?by?chymotrypsin?alone?or?in?combination?with?other?proteases.?Suitable?for?peptide?
mapping,?fingerprinting,?and?sequence?analysis.
For?peptide?digestion,?use?a?ratio?(w/w)?of?approximately?1:60?for?chymotrypsin:peptide.?Perform?peptide?
digests?in?100?mM?Tris?HCl?containing?10?mM?CaCl2,?pH?7.8,?at?30?°C.?Self?digestion?may?occur?if?
temperatures?above?37?°C?are?used.?A?known?peptide?such?as?melittin?should?be?used?as?a?control?for?all?
experiments.
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MAIN?FEATURES?
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Source | E.?Coli |
Purified?by | HPLC? |
Physical?form | White?lyophilized?powder |
Specific?activity | ≥1000?unit/mg?pro |
Purity | >?99% |
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PRODUCT?INFORMATION
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Product | Cat.?No. | Specific?activity | Packaging | Manufacturer |
Recombinant?Chymotrypsin | RCT10 | ≥1000?unit/mg?pro | 1mg,100mg,1g?or?bulk | YaxinBio |
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UNIT?DEFINITION:?One?unit?will?hydrolyze?1.0?μmole?of?BTEE?per?min?at?pH?7.8?at?25?°C.
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RELATED?PRODUCT
Recombinant?Trypsin
V8
?
Shanghai Yaxin Biotechnology Co.,Ltd.
Address: No.466 YinDu Road,XuHui, Shanghai, China, Shanghai, Shanghai, China, 200000
Tel: 86-021-54336592
Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise
that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only
a company on researching and producing the recombinant carboxypeptidase B and recombinant
trypsin. Animal original free enzymes are specially used in the human recombinant insulin production, and exporting abroad.
Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the
usage of trypsin in the cell culture.Animal original free is very important for the production of
antibody and vaccine Usage of recombinant trypsin fundamentally solves the problem of animal original virus contamination
in the human recombinant insulin production.
YaxinBio insists on 鈥渟cientific and technological innovation鈥?has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant
carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement
transformation project in Sep. 2013.
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