Agrochemicals & Pesticides

Recombinant Enterokinase or Recombinant Bovine Enterokinase ?EC 3.4.21.9

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Description

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Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.

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Advantages

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1) Protease that cleaves specifically after a lysine preceded by four aspartic acids:

Asp-Asp-Asp-Asp-Lys (DDDDK)

2) No any other contaminated proteases, no non-specific cutting sites.

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Recommend Usage Condition:

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Cutting condition:?????????????given an example: 25mM Tris-HCl 8.0

Fusion protein concentration:??0.1-1mg/ml (total protein content: 0.5-1.0mg)

EK content:???????????????????1-2U

Temperature:??????????????????25℃

Time:?????????????????????????overnight or 12h-16h for digestion.

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Common Components Influence the Action of Enterokinase

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＞200mM imidazole or ＞200mM NaCl or ＞5%glycerin, the reaction may be effected.The

following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please?dilute the sample to ＜100mM imidazole, ＜50mMNaCl,

＜5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase

the content of EK in reaction system or extend the reaction time.

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Main Features

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M.W. | 25,850 Da ? |
Specific Activity | One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in?25mMTris-HCl, pH 8.0.?Substrate: a special fusion protein.? |
Stability | Keep cool with blue ice during shipping.?Remained stable at 25°C for one week?without activity lost.?No activity lost after 5 cycles?of frozen-thawing. |
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Product Information

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Product | ?Activity | Packaging | Manufacturer |
Recombinant?Enterokinase | ≥5 u/μl | 100u;500u;1ku; 10ku;20ku;100ku | YaxinBio |
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