Chemical Reagent

Carboxypeptidase B catalyzes hydrolysis of the basicamino acids lysine, arginine and histidine from the Cterminal of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B (EC 3.4.17.2) is expressed in E.Coli and purified by high pressure liquid chromatography. There is no foreign enzyme activity such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF contain in preparation.
ADVANTAGES
Animal origin free
Recombinant carboxypeptidase B eliminates the risk of viruses, or other potential adventitious agents found in animal derived carboxypepitdase B. Which belong to the AOF level 3 products.
Stable
A sterile recombinant carboxypeptidase B lyophilized powder eliminates the contamination opportunity and decreases the chance of activity loss in the process of transport and storage. Convenient for transport and store.
High purity
Recombinant carboxypeptidase B provides increased specificity and eliminates contaminating activities found in lower purity enzymes One single main band at 35kD by SDS-PAGE.
Protein content 35% ～ 50%
Special activity >170 units/mg pro.
Purity > 95%
Foreign activity No chymotrypsin, carboxypeptidase A, and other protease contaminant.
The content of recombinant trypsin is less than 10ppm.
PRODUCTS INFORMATION
Product Cat. No. Special activity Packaging Manufacturer
Recombinant Carboxypeptidase B RCPB01170 ≥170 u/mg pr10mg, bulk YaxinBio
RCPB01100 ≥100 u/mg pro 1g, bulk YaxinBio
UNIT DEFINITION：One Unit of