Chemicals Stocks

Recombinant Carboxypeptidase B E.coli ? Determine C-terminal Amino Acids

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Description

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Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli?and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

CAS: 9025-24-5
EC: 3.4.17.2

Source: Expressed in E. Coli.

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Advantages

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Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific?activity?and?eliminates contaminating proteases activities found in extracted enzymes with lower purity?level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.

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Main Features

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Format | White or white-like?or yellowish?lyophilized |
Additives | Tris salts, NaCl salts and carbohydrates |
Protein content | 35% ～ 60% |
Purity | Single main band on SDS-PAGE |
M.W. | 33.8kD |
Contaminant activity | Less than 10 ppm of recombinant trypsin. |
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Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of???

???????????????????????????????? hippuryl-L-arginine per minute at 25℃, pH 7.65.

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Recommend Usage

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To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH?7-9.

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Storage Instructions

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Recommend recombinant carboxypeptidase B lyophilized?should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90%?activity remained?after 10 times repeated freezing and thawing.

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Email:Zhaozhi@yaxinbio.com