Bio-technology Products

Pullorum Disease (PD) and Fowl Typhoid (FT) Antibody ELISA kit
Catalog No. LSY-30027

1. Usage
This kit is used to detect Pullorum Disease (PD) and Fowl Typhoid (FT) antibody in chicken serum, to assess antibody condition by Pullorum Disease (PD) and Fowl Typhoid (FT) vaccine in chicken farm and assist diagnosis of serological infected chicken.

2. Principle
The Pullorum Disease (PD) and Fowl Typhoid (FT) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).

3. Reagents
1)Antigen coated microplate 1 piece (96 wells)
2)Enzyme conjugate 1 bottle (12 mL/bottle)
3)10×concentrated washing buffer…………………………1 bottle (50 mL/bottle)
4)Substrate A /B 1 tube each (6 mL/tube)
5)Sample diluent……………………………………………. 1 bottle (50 mL/bottle)
6)Stop solution 1 tube (6 mL/tube)
7)Negative control serum 1 tube (800μL /tube)
8)Positive control serum 1 tube (800μL /tube)
9)Serum diluent plate……………………………………………………..…..1 piece
10)Adhesive film……………………………………………………………….2 pieces
11)Instruction……………………………………………………………………1 piece

4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
2) 37 ℃ Incubator
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine

5. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, without hemolysis.