Bio-technology Products

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Antibody ELISA kit
Catalog No. LSY-30010
1. Brief
The GreenSpring? Porcine reprodutive and respiratory syndrome virus antibody test kit (PRRSV Ab) is used for the detection of PRRSV antibodies in swine serum; assessment of immunity conditions against porcine reprodutive and respiratory syndrome virus in the pig farms; and investigation epidemiology of the PRRSV.

2. Principle
The GreenSpring? PRRSV Ab ELISA test kit is made from the antigen coated microtiter plate, goat-anti-pig IgG-HRP and other reagents. It applies the indirect ELISA principle to test the antibodies against PRRSV in porcine serum. In the test, the coated antigen combine with PRRSV-IgG in serum, then add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the PRRSV-IgG.

3. Components
1PRRSV Antigen Coated Microplate 2 plates (96 wells)
2PRRSV-IgG Negative control (green lid) 1 tube (1.5 mL)
3PRRSV-IgG Positive control (red lid) 1 tube (1.5 mL)
4PRRSV Enzyme Conjugate (yellow lid) 1 bottle (22 mL)
5Wash Concentrate (20×) (white lid) 1 bottle (50 mL)
6Substrate A (purple lid)/B (orange lid) 1 bottle each (12 mL/bottle)
7Stop solution (blue lid) 1 bottle (12 mL)
8Sample diluent solution (transparent lid) 1 bottle (50 mL)
9 Adhesive Foil 6 pieces
10 Instruction 1 piece
(Note: Never mix up reagents from test kits in different batch number)

4. Material Required not Provided
1Microplate Reader (single-wave length: 450nm or double-wave length: 450/630 nm).
2Microplate Washer.
3Micropipettes, adjustable.
4Constant temperature box or water bath box.
5Oscillator.