Porcine Circovirus (PCV2) ELISA test Kit - LSY-30007

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Porcine Circovirus (PCV2) ELISA test Kit

Porcine Circovirus (PCV2) ELISA test Kit
Catalog No. LSY-30007
1. Usage
The GreenSpring? porcine circovirous ELISA Test kit is used for detection of porcine circovirus antibodies in the serum; assessment of immunity conditions against porcine circovirus in the pig farm and investigation of the epidemiology of the porcine circovirus.

2. Principle
The GreenSpring? PCV2 ELISA kit is based on an indirect enzymatic immunoassay(Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by ELISA Reader.

3. The kit components
1 PCV Antigen coated microtiter plate 2 pieces (96 wells)
2 Negative (green lid)/positive (red lid)serum 1 tube each (1.5 mL/tube)
3 Enzyme conjugate (yellow lid) 1 bottle (22 mL/bottle)
4 20×concentrated washing buffer (white lid) 1 bottle (50 mL/bottle)
5 Substrate (orange lid) 2 bottles (12 mL/bottle)
6 Stop solution(blue lid) 1 bottle (12 mL/bottle)
7 Sample diluent solution(transparent lid) 1 bottle (50 mL/bottle)
8 Adhesive Foil 6 pieces
9 Instruction 1 piece

4. Materials Required But Not Provided
1Microplate Reader (double-wave length: 450/630 nm).
2Precise micropipette (single-channel 1-100ul、0.5-10ul、multi-channel 30-300ul)
3Constant temperature box or water bath box.
4Oscillator.
5Microplate Washer.
6Disposable tips (10ul, 200ul)
7 Deionized water

5. Sample requirement
1The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.
2Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
3The EDTA, heparin sodiun and other anticoagulants will not affect the results.

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Shenzhen Lvshiyuan Biotechnology Co.,Ltd

Address: D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Longgang, Shenzhen,518120, China, Shenzhen, Guangdong, China, 518120
Tel: 86-755-28438788
Shenzhen Lvshiyuan Biotechnology Co.,Ltd is a new and high technology enterprise, founded by a well-known senior scholars from USA in 2003. We specialize in the most advanced Food Safety ELISA test kit, Animal disease diagnostic kits, small molecule antigen and antibodies, gene recombination antigen and antibodies, etc. and are currently immersed in an ambitious international development process. Dedication and commitment, along with a variety of individual strengths and talents, make each of the employees at Lvshiyuan a real asset to the organization. Our scientific staff has experience with medical, industrial and environment diagnostics. We are able to customize programs to serve client鎶?specific needs with competitive pricing and high quality standards. Shenzhen Lvshiyuan Biotechnology Co.,Ltd is one of the largest and most complete manufacturers on biological diagnostic reagents in China. We firstly commercialize Nitrofurazone, Nitrofurantoin metabolites ELISA test kit and other products in the world. Our products are widely used in government labs, enterprises and research institutes. To meet the needs of market, we are developing hundreds of kits. We take 鎻僽ilding up Green world, Spring from you and our cooperation?as the goal insist that 鎻僫otechnology serves humanity?as the management ideal, provide the leading biological technology products and the perfect technical service for the customers.

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