Biochemical

Oxytetracycline ELISA Diagnostic Kit
EU preliminary provisions total muscle and milk tetracycline compounds shall not exceed 100ppb,

in the kidney, liver and eggs respectively shall not exceed 600 ppb, 300 ppb and 200ppb. The OTC

(Oxytetracycline) ELISA kits utilizing the latest biotechnology development has easy, rapid, and sen

sitive advantages.



This kit uses indirect-competition method mainly based on the immunological reaction between

specific antigen and antibody. Oxytetracycline specific antigen coated on microtiter plates competes

binding sites of enzyme-labeled Oxytetracycline specific antibody with added calibrator (or sample).

With TMB chromogenic substrate, the absorbance value was negatively correlated with residues

of Oxytetracycline. Under the calibration curve, Oxytetracycline content of the sample can be quantified

multiplied by the corresponding compare dilution.



Specificity

Oxytetracycline------------------------------------------------100%

Tetracycline----------------------------------------------------200%

Chlortetracycline----------------------------------------------200%

doxycycline (doxycycline) -----------------------------------20%

Detection limit:

0.4ppb is the lowest detection limit of Oxytetracycline kit.

Linear regression is between 0.4-10.8ppb.



Sensitivity:

Tissue ------------------------------------------------------6ppb



Recovery rate:

Tissue--------------------------------------------------75-105%