Chemical Projects

Oryza Sativa Human Serum Albumin 66.5kD With Less Than 0.25EU/mg Endotoxin ?

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Quick detail
# # CAS No.: 70024-90-7
# Other Names: rHSA
# EINECS No.: 274-272-6
# Brand Name: Oryzogen
# Model Number: HY100M1
# Formulation: OsrHSA is lyophilized with saline
# Endotoxin: Less than 0.25EU/mg
# Storage and Handling: Stored at temperatures 2-8℃ for 24 months


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Physiological and Biochemical Properties of rHSA and pHSA

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Char. | pHSA | rHSA |
Amino Acids Sequence | Same | Same |
N-terminus | DAHKSEV | DAHKSEV |
C-terminus | KLVAASQAALGL | KLVAASQAALGL |
Glycosylation | No | No |
Molecular Mass(MALDI) | 66.554KD | 66.550KD |
PI | 4.8 | 4.8 |
Drug Binding | Similar | Similar |
Thermal Stability | M/P 65℃ | M/P 65℃ |
Lipid Conjugation | Identical | Identical |
Crystal Structure | Identical | Identical |
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Description

Compared with the HSA from plasma, rHSA amino acid sequence entirely. The the secondary and tertiary structure of OsrHSA are identical to those of pHSA. Spectroscopic analysis further confirmed that OsrHSA has the same conformation as pHSA. In terms of immunogenicity and protein activity, rHSA is superior to pHSA and can be used in various fields instead of pHSA.

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Advantages?

1.?Healthgen recombinant human serum albumin is free of animal origin, which effectively avoid any potential risk of animal bacteria, prions, virus and contagious protein disease.

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2.?Healthgen Biotechnology Corp. has been committed in research of protein purification and meanwile our technology has been patented by the US Patent and Trademark Office on May 15th 2015. & quot;A method for isolating and purifying recombinant human serum albumin from transgenic rice grain& quot;. The patent number is US9, 023,990. The purity of our rHSA is very high. Now, our rHSA purity can be as high as 99%.

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3.?Healthgen recombinant human serum albumin (rHSA) has excellent batch consistency. Using self-pollination rice as the bioreactor to express the rHSA, with the world& acute;s advanced facility and top production proceeding to extract and purify the rHSA, Healthgen can ensure the excellent consistency of rHSA from different batches.?

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Supplemental Description

Human serum albumin has been widely used as plasma expansion reagent. Because of its non-enzymatic activity and no immunogenicity, etc. HSA can also be used as excipient and stabilizer in a variety of pharmaceutical preparations and serum-free cell culture medium component. In addition, HSA is also used as a drug carrier due to its antioxidant properties, thereby conferring drugs better physicochemical properties. At present, most HSA products in the market are directly derived and refined from human plasma.

On the one hand, due to the potential existence of various infectious disease pathogens in the human blood, especially AIDS, hepatitis B and other viruses. Thereby, these virus from human plasma will be brought with HSA to the final products. rHSA produced by gene recombinant technology can effectlively avoid the risks of virus pollution and infection. What’s more, the supply of human blood is limited. Therefore, it’s very urgent to search blood substitutes. The establishment and development of recombinant DNA technology provide an important method for the acquisition of biological materials. In recent years, many researchers have been hotly committed to the recombinant human serum albumin research.

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