Inorganic Chemicals

No Fragments / Polymers Human Serum Cell Culture Keep Stem Cell Undiferentiation ?

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Quick detail
# # CAS No.: 70024-90-7
# Other Names: rHSA
# EINECS No.: 274-272-6
# Place of Origin: Hubei, China (Mainland)
# Brand Name: Oryzogen
# Model Number: HY100M1
# Purity: Greater than 99 % as determined by SDS-PAGE
# Source: Rice Grain (Oryza Sativa)
# Physical Appearance: Off-white to light beige lyophilized powder
# Formulation: OsrHSA is lyophilized with saline
# Endotoxin: Less than 0.25EU/mg
# Storage and Handling: Stored at temperatures 2-8℃ for 24 months


Physiological and Biochemical Properties of rHSA and pHSA

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Char. | pHSA | rHSA |
Amino Acids Sequence | Same | Same |
N-terminus | DAHKSEV | DAHKSEV |
C-terminus | KLVAASQAALGL | KLVAASQAALGL |
Glycosylation | No | No |
Molecular Mass(MALDI) | 66.554KD | 66.550KD |
PI | 4.8 | 4.8 |
Drug Binding | Similar | Similar |
Thermal Stability | M/P 65℃ | M/P 65℃ |
Lipid Conjugation | Identical | Identical |
Crystal Structure | Identical | Identical |
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Description?

Stem cells are pluripotency and will gradually differentiate into different functional cells during cell culture. In order to maintain the pluripotency of stem cells, the stem cell medium can not contain any animal components or some unknown substance. rHSA can be used as a medium supplement in stem cell culture process to help stem cells to maintain versatility. Because rHSA is a recombinant human serum derived from rice grains, and doesn& acute;t contain any animal component, which is much safer than plasma derived HSA.





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Advantages

1.?The chairman of Healthgen Professor Daichang.Yang is one of China& acute;s chief scientists. Professor Yang has rechieved the “Thousand Talents program” of the National Central Organization Department, & quot;Hundred Talents Program& quot; of the Organization Department of Hubei Province and Top 10 & quot;Venture Eagle& quot; entrepreneurs.

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2. With years of effort, Healthgen has developed the State-of-the-Art platform called OryzHiExp, having high capacity to express no animal origin proteins and peptides in the rice endosperm cells.

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3.?The rice endosperm specific expression platform is easy to be large-scale. The rice endosperm has the protein body, which can keeps proteins stability and bioactivity. At present, the production capacity is almost 1000 kg / year.

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Supplemental Description

The research results have been granted with 16 international patents, covering countries, such as United States, Japan, Europe, Canada and South Africa. Parts of the patents are showed as below:

A callus-specific promoter of rice and the use thereof

China patent no. 201110054109.X

Method for expressing small peptides using cereal non-storage proteins as fusion carrier in endosperm and the use thereof

US patent no. US20070289033

Japan patent no. JP2007-325594A

Europe patent no. EP1865064

Canada patent no. CA2587092

China patent no. 200610019285.9

A method for extracting recombinant human serum albumin from transgenic rice grain

South Africa patent no. 2013/04359

China patent no. 201010597544.2

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain;

South Africa patent no. 2013/04358

China patent no. 201010606635.8

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain

South Africa patent no. 2013/04358

US patent no. US9023990 B2

Production of recombinant human serum albumin with rice endosperm cells as biological reactor

China patent no. ZL 2005 1 0019084.4

Based on the innovative technology, Healthgen also published research papers.

Publications

1. He Y, et al. Large-scale production of functional human serum albumin from transgenic rice seeds. Proc Natl Acad Sci USA. 2011;108:19078-19083.

2. Na An, et al. Expression of a Functional Recombinant Human Basic fibroblast Growth Factor from Transgenic Rice Seeds. Int. J. Mol. Sci. 2013, 14, 3556-3567

3. Liping Z, et al. Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed. Journal of Biotechnology. 2012; 164: 300-308.

4. Xie, T, et al. A biologically active rhIGF-1 fusion accumulated in transgenic rice seeds can reduce blood glucose in diabetic mice via oral delivery. Peptides 2008: 29: 1862-1870.

5. Zhen Chen, et al. Human serum albumin from recombinant DNA technology: challenges and strategies. Biochimica et Biophysica Acta. 2013; 1830: 5515-5525.

6. Zhen Chen, et al. Quantitation of the residual DNA from rice-derived recombinant human serum albumin. Analytical Biochemistry. 2014; 450: 4-10.

7. Jiquan Ou, et al. Transgenic rice endosperm as a bioreactor for molecular pharming. Plant Cell Rep (2014) 33:585–594

8. Kai fu, et al. Immunotoxicity assessment of rice-derived recombinant human serum albumin using human peripheral blood mononuclear cells. PLoS ONE 01/2014; 9(8): e104426.