Chemicals Agents

Product Description

REAGEN? Nitrofurazone (SEM)) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Nitrofurazone in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.
The unique features of the kit are:
? high recovery (75%-105%), and cost-effective extraction methods for various samples.
? High sensitivity (0.05 ng/g or ppb) and low detection limit (0.1 ng/g or ppb) for various samples.
? High reproducibility.
? A quick ELISA assay (less than 1.5hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The SEM antibody has been coated in the plate wells. During the analysis, sample is added along with the SEM-horseradish peroxidase (SEM-HRP) conjugate. If the SEM residue is present in the sample, it will compete for the SEM antibody, thereby preventing the SEM-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the SEM residue concentration in the sample.
Required Materials Not Provided With the Kit
? Microtiter plate reader (450 nm)
? Incubator
? Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
? Rotary evaporator or nitrogen gas
? Vortex mixer (e.g. Gneie Vortex mixer from VWR)

? 10, 20, 100 and 1000 ?L pipettes
? Multi-channel pipette: 50-300 ?L (Optional)
? Ethyl acetate
? 0.1 M K2HPO4
? n-Hexane (or n-Heptane)