Biochemical

Ivermectin is antiparasitic drug with potential neurotoxicity in animals with characteristic of
long residence time. World Health Organization be classified as highly toxic compound.
Ivermectin ELISA kits utilizing the latest biotechnology development has easy, rapid, and
sensitive advantages.

This kit uses indirect-competition method mainly based on the immunological reaction
between specific antigen and antibody. Ivermectin specific antigen coated on microtiter
plates competes binding sites of enzyme-labeled Ivermectin specific antibody with added
calibrator (or sample). With TMB chromogenic substrate, the absorbance value was negatively
correlated with residues of Ivermectin. Under the calibration curve, Ivermectin content of the
sample can be quantified multiplied by the corresponding compare dilution.

Detectable samples
Ivermectin residue can be quantitative of tissue and milk.

Specificity
Ivermectin-------------------------------------------------------100%
avermectin------------------------------------------------------380%
eprinomectin ----------------------------------------------------50%
doramectin ------------------------------------------------------30%
Detection limit:
2.5ppb is the lowest detection limit of Ivermectin kit. Linear regression is between 2.5-67.5ppb.

Sensitivity:
Tissue-----------------------------------------------------20ppb
Milk-----------------------------------------------------------5ppb

Recovery rate:
Tissue -------------------------------------------------60-120%
Milk-----------------------------------------------------60-120%