Bio-technology Products

Infectious Bronchitis IgG (IBV) antibody ELISA kit
Catalog No. LSY-30017
1. Introduction
The GreenSpring? Infectious Bronchitis IgG (IBV) antibody ELISA kit is developed to detect the IBV antibodies level in chicken serum sample and can be used to evaluate serological diagnosis of infected chickens and Epidemiological surveys of Infectious Bronchitis virus, analysis of Infectious Bronchitis virus vaccine status in chickens.

2. Principle
This kit is based on solid-phase enzyme-linked immunosorbent assay (ELISA) principle, composed by the reaction Micro-plate coated with high purity IBV antigen, horseradish peroxidase-labeled anti-chicken IgG and other reagents. The reaction mechanism is the coated antigen binding with IBV-Ab in sample, and then with the enzyme-labeled anti-chicken IgG antibody to form a & quot;coated antigen + IBV-Ab + anti-chicken IgG HRP antibody& quot; complex, add substrate, it will have coloration by the enzyme catalytic reaction. Color depth is proportional to the amount of IBV-Ab, when the sample chromogenic reaction, the results detected by the microplate reader exceeds a set threshold value result judged as positive, indicating that the immune produced antibodies or natural infection exists.

3. The kit components
1 IBV antigen coated microplate 96T X2
2 Enzyme conjugate 25 ml Yellow lid
3 Sample diluent 50 ml transparent lid
4 IBV-IgGNegative control serum 1.5 ml green lid
5 IBV-IgG Positive control serum 1.5 ml red lid
6 Substrate 12*2 ml orange lid
8 Stop solution 12 ml blue lid
9 20×concentrated washing buffer 50 ml white lid
10 Adhesive Foil 6 pieces
11 Instruction 1 piece

4. Materials Required But Not Provided
1Microplate Reader (double-wave length: 450/630nm).
2Micropipettes: single-channel 1~100l,0.5~10l, multi-channel 30~300l
3Microplate Washer.
4Water bath or incubator.
5Oscillator.
6Disposable tips (10ul, 200ul)
7Deionized water