Bio-technology Products

Hog Cholera Virus Antibody ELISA Test Kit
Catalog No. LSY-30006
1. Brief
The GreenSpring? hog cholera virus antibody test kit, or called Classical Swine Fever Virus Antibody ELISA Test Kit (CSFV Ab), is used for the detection of hog cholera virus antibodies in porcine serum; assessment of immunity conditions against hog cholera virus in the pig farms and epidemiology investigation of the hog cholera.

2. Principle
The GreenSpring? Classical Swine Fever Virus Antibody ELISA Test Kit is made from the antigen coated microtiter plate, goat-anti-pig IgG-HRP and other reagents. It applies the indirect ELISA principle to test the antibodies against Classical Swine Fever virus in porcine serum. In the test, the coated antigen combine with CSFV-IgG in serum, then add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the CSFV-IgG, when the value of color is greater than the cut-off value, the pigs are vaccinated well.

3. Components
1 CSFV antigen coated microplate 2 pieces (96 wells)
2 CSFV Negative serum(green lid) 1 tube (1.5 mL)
3 CSFV Positive serum (red lid) 1 tube (1.5 mL)
4 CSFV Enzyme conjugate (yellow lid) 1 bottle (22 mL)
5 20×Wash Concentrate (white lid) 1 bottle (50 mL)
6 Substrate A (purple lid)/B (orange lid) 1 bottle each (12 mL/bottle)
7 Stop solution(blue lid) 1 bottle (12 mL)
8 Sample diluent solution(transparent lid) 1 bottle (50 mL)
9 Adhesive Foil 6 pieces
10 Instruction 1 piece
(Note: Never mix up reagents from test kits in different batch number)

4. Material Required But not Provided
1Microplate Reader (single-wave length: 450nm or double-wave length: 450/630 nm).
2Microplate Washer.
3Micropipettes, adjustable.
4Constant temperature box or water bath box.
5Oscillator.

5. Sample requirement
1The samples are porcine serum, which should be collected with no bacteria. The storage time should be les