For Enzymatic Hydrolysis of Protein，CAS 9002-07-7 / Protein sequencing - RPT0201
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for Enzymatic Hydrolysis of Protein,CAS:9002-07-7,Protein sequencing
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Bulk Recombinant Trypsin For Manufacturing Recombinant Insulin
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DESCRIPTION
Recombinant Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as from carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
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ADVANTAGES
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stable:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1. Recombinant porcine trypsin provides increased specificity and eliminates contaminating activities found in enzymes with lower purity level.
2. No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3. One single main band at 24kD by SDS-PAGE.
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MAIN?FEATURES?
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Source | E.?Coli |
Purified?by | HPLC |
Physical?form | White?lyophilized?powder |
Additives | Carbohydrates |
Protein?content | 35%?～?70% |
Specific?activity | ≥3800?USP?units/mg?pro |
Purity | >?99% |
Contaminant?activity | No?chymotrypsin,?carboxypeptidase?A,?and?other?protease?contaminant. |
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PRODUCT?INFORMATION
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UNIT?DEFINITION:One?USP?unit?of?trypsin?activity?will?produce?a?Delta?A253?of?0.003?per?minute?in?a?
???????????????????????????? reaction?volume?of?3.0ml?at?pH7.6?and?25℃,?with?BAEE?as?a?substrate?(1cm?light?path).
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Product | Cat.?No. | Specific?activity | Packaging | Manufacturer |
Recombinant?Porcine?Trypsin | RPT0201 | ≥3800?USP?u/mg?pro | 10mg,100mg,1g?or?bulk | YaxinBio |
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RELATED?PRODUCT
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Recombinant?Carboxypeptidase?B
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Shanghai Yaxin Biotechnology Co.,Ltd.
Address: No.466 YinDu Road,XuHui, Shanghai, China, Shanghai, Shanghai, China, 200000
Tel: 86-021-54336592
Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise
that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only
a company on researching and producing the recombinant carboxypeptidase B and recombinant
trypsin. Animal original free enzymes are specially used in the human recombinant insulin production, and exporting abroad.
Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the
usage of trypsin in the cell culture.Animal original free is very important for the production of
antibody and vaccine Usage of recombinant trypsin fundamentally solves the problem of animal original virus contamination
in the human recombinant insulin production.
YaxinBio insists on 鈥渟cientific and technological innovation鈥?has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant
carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement
transformation project in Sep. 2013.
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