Foot-and-mouth disease virus (FMDV) Type O Antibody ELISA Test Kit , China Foot-and-mouth disease virus (FMDV) Type O Antibody ELISA Test Kit Supplier

Foot-and-mouth disease virus (FMDV) Type O Antibody ELISA Test Kit - LSY-30031

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Foot-and-mouth disease virus (FMDV) Type O Antibody ELISA Test Kit
Catalog No. LSY-30031
1.Principle
This kit is used to detect specific antibody against foot and mouth disease virus (FMDV) Type O Antibody in serum of bovine、goat and porcine qualitatively, for monitoring antibody after FMDV Type O immune.
This kit use block ELISA method, FMDV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code Item Spec. Code Item Spec.
1 Antigen Coated plates 96 wells 6 Stop solution 6 ml
2 Enzyme Conjugate 11 ml 7 Positive control 0.5 ml
3 10X Washing buffer 50 ml 8 Negative control 1 ml
4 Substrate 11 ml 9 Adhesive Foil 2 pieces
5 Sample diluent 50 ml 10 Instruction 1 piece
3. Material required not provided
1) Micropipette: 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450nm wavelenth.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis

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Shenzhen Lvshiyuan Biotechnology Co.,Ltd

Address: D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Longgang, Shenzhen,518120, China, Shenzhen, Guangdong, China, 518120
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Shenzhen Lvshiyuan Biotechnology Co.,Ltd is a new and high technology enterprise, founded by a well-known senior scholars from USA in 2003. We specialize in the most advanced Food Safety ELISA test kit, Animal disease diagnostic kits, small molecule antigen and antibodies, gene recombination antigen and antibodies, etc. and are currently immersed in an ambitious international development process. Dedication and commitment, along with a variety of individual strengths and talents, make each of the employees at Lvshiyuan a real asset to the organization. Our scientific staff has experience with medical, industrial and environment diagnostics. We are able to customize programs to serve client鎶?specific needs with competitive pricing and high quality standards. Shenzhen Lvshiyuan Biotechnology Co.,Ltd is one of the largest and most complete manufacturers on biological diagnostic reagents in China. We firstly commercialize Nitrofurazone, Nitrofurantoin metabolites ELISA test kit and other products in the world. Our products are widely used in government labs, enterprises and research institutes. To meet the needs of market, we are developing hundreds of kits. We take 鎻僽ilding up Green world, Spring from you and our cooperation?as the goal insist that 鎻僫otechnology serves humanity?as the management ideal, provide the leading biological technology products and the perfect technical service for the customers.