Fluorescent In Situ hybridization (FISH) - 333333
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Fluorescence in situ hybridization (FISH) techniques allow specific nucleic acid sequences to be detected in morphologically preserved chromosomes, cells or tissue sections. In combination with immunocytochemistry, Fluorescence in situ hybridization can relate microscopic topological information to gene activity at the DNA, mRNA, and protein level. FISH has become a molecular diagnostic technique utilizing fluorescently labeled DNA probes to detect or confirm gene or chromosome abnormalities. The sample DNA (metaphase chromosomes or interphase nuclei) is first denatured, a fluorescently labeled probe of interest is then added to the denatured sample mixture and hybridizes with the sample DNA at the target site as it re-anneals back into a double helix. The probe signal can then be seen through a fluorescent microscope and the sample DNA can be scored for the presence or absence of the signal. Unlike most other techniques used to study chromosomes, FISH does not have to be performed on cells that are actively dividing. This makes it a very versatile procedure. This technique is useful for identifying chromosomal abnormalities, gene mapping, characterizing somatic cell hybrids, identifying amplified genes and studying the mechanism of rearrangements. RNA ISH is used to measure and localize mRNAs and other transcripts within tissue sections or whole mounts.
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Creative Bioarray is a well-recognized service provider propelled by integrity, expertise and the desire to contribute to simplify, improve, and accelerate research speed of the scientists by providing the highest standard quality research products and services. Since it founded, Creative Bioarray had recognized that an important obstacle to make development of basic bioscience and preclinical researches using human specimens is the lack of access to reference materials. Consequently, Creative Bioarray was determined to be the first commercial entity specialized in building up the largest tissue-sharing platform by integrating the detailed clinical information of all the tissue samples.
Creative Bioarray is continually improving and expanding their products and services line to meet the growing demand of scientists in the biomedical science research. Collaborating with biotechnology and pharmaceutical companies as well as hospitals and academic research laboratories all over the world, Creative Bioarray has established one of the largest commercially available bank of paraffin embedded and frozen tissues to meet specific research requirements from different clients. For details, Creative Bioarray has collected information from over 3,000 tissue banks or tissue reservoirs/ depositories in USA, Europe, Asia like China, Korea and other areas of the world, has built up a database that contains over 500,000 human tissue samples and 30,000 animal tissue
