Fluorescent In Situ hybridization (FISH) - 161616

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Fluorescent In Situ hybridization (FISH)

Fluorescence in situ hybridization (FISH) is a molecular diagnostic technique utilizing fluorescently labeled DNA probes to detect or confirm gene or chromosome abnormalities. The sample DNA (metaphase chromosomes or interphase nuclei) is first denatured, a fluorescently labeled probe of interest is then added to the denatured sample mixture and hybridizes with the sample DNA at the target site as it re-anneals back into a double helix. The probe signal can then be seen through a fluorescent microscope and the sample DNA can be scored for the presence or absence of the signal. Unlike most other techniques used to study chromosomes, FISH does not have to be performed on cells that are actively dividing. This makes it a very versatile procedure.

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