Biochemical

Product Description

REAGEN? Dioxin ELISA Test Kit is a indirect immunoassay (ELISA) for the quantitative analysis of Dioxin in fish, shrimp,soil and water. If necessary, positive samples can be confirmed by instrumental analysis or other conventional methods.

The unique features of the kit are:
? High recovery (75-105%), and cost-effective extraction methods .
? High sensitivity (2.5 pg/g or ppt)
? Quick ELISA assay (less than 3 hours regardless of the number of samples)
? High reproducibility.

Procedure Overview

The test is an indirect competitive ELISA that allows the sensitive detection of Dioxins .It is based on the recognition of Dioxins congeners by specific antibodies. Dioxins when present in a sample and a Dioxin-protein analogue immobilized on the plate compete for the binding sites of antibodies in solution. After a washing step, a second antibody-HRP label is added. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of the Dioxin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader.