Biochemical

Diethylstilbestrol (DES) is effective anabolic steroids, coupled with estrogen activity. Poultry or fish in
excess of diethylstilbestrol residues might cause harm to consumers. Diethylstilbestrol has been banned
in food production applications. The EU demanded 2ppb-positive limit. The Diethylstilbestrol ELISA kits
utilizing the latest biotechnology development has easy, rapid, and sensitive advantages.

This kit uses indirect-competition method mainly based on the immunological reaction between specific
antigen and antibody. Diethylstilbestrol specific antigen coated on microtiter plates competes binding
sites of enzyme-labeled Diethylstilbestrol specific antibody with added calibrator (or sample). With TMB
chromogenic substrate, the absorbance value was negatively correlated with residues of
Diethylstilbestrol. Under the calibration curve, Diethylstilbestrol content of the sample can be quantified
multiplied by the corresponding compare dilution.

Detectable samples
Diethylstilbestrol residue can be quantitative of tissue and urine.

Specificity
Diethylstilbestrol-----------------------------------------------100%
Detection limit:
0.1ppb is the lowest detection limit of Diethylstilbestrol kit. Linear regression is between 0.1-2.7ppb.

Sensitivity:
Tissue ----------------------------------------------------0.8ppb
Liver ------------------------------------------------------1.5ppb
Urine (Method 1)-----------------------------------------2ppb
Urine (Method 2)-----------------------------------------1ppb

Recovery rate:
Tissue--------------------------------------------------75-105%
Liver----------------------------------------------------75-105%
Urine---------------------------------------------------75-105%