Biochemical

Dexamethasone, anti-inflammatory and anti-allergy, primarily is a first aid medication of critical illness
and various inflammation treatment. Children medication must be very careful because it inhibits the
growth and development of children. If it is necessary to long-term use, short-acting or
intermediate-acting formulations should used to avoid the use of long-acting formulations of
dexamethasone. Geriatric medicine is prone to hypertension, especially post-menopausal women is
prone to osteoporosis. Dexamethasone ELISA kits utilizing the latest biotechnology development has
easy, rapid, and sensitive advantages.
This kit uses indirect-competition method mainly based on the immunological reaction between specific
antigen and antibody. Dexamethasone specific antigen coated on microtiter plates competes binding
sites of enzyme-labeled Dexamethasone specific antibody with added calibrator (or sample). With TMB
chromogenic substrate, the absorbance value was negatively correlated with residues of
Dexamethasone. Under the calibration curve, Dexamethasone content of the sample can be quantified
multiplied by the corresponding compare dilution.

Detectable samples
Dexamethasone residue can be quantitative of tissue.

Specificity
Dexamethasone-----------------------------------------------100%
triamcinolone ---------------------------------------------------45%
prednisolone ---------------------------------------------------10%
betamethasone ------------------------------------------------20%
Detection limit:
0.015ppb is the lowest detection limit of Dexamethasone kit. Linear regression is between 0.015-1.215ppb.

Sensitivity:
Tissue (Method 1)-------------------------------------0.2ppb
Tissue (Method 2)-------------------------------------0.4ppb

Recovery rate:
Tissue -------------------------------------------------70-110%