Chemicals Agents

Product Description

REAGEN? Clonidine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Clonidine in fish, shrimp, meat, feed and urine. The unique features of the kit are:
? High recovery (75-105%), rapid, and cost-effective extraction methods
? High sensitivity (0.015 ng/g or ppb)
? High reproducibility
? A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Required Materials Not Provided With the Kit

? Microtiter plate reader (450 nm)
? Incubator
? Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
? Rotary evaporator or nitrogen gas
? Vortex mixer (e.g. Gneie Vortex mixer from VWR)
? 10, 20, 100 and 1000 ?L pipettes
? Multi-channel pipette: 50-300 ?L (Optional)