Chloramphenicol(CAP) ELISA Test Kit - LSY-10007

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Chloramphenicol(CAP) ELISA Test Kit

Chloramphenicol ELISA test Kit
Catalog No. LSY-10007
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 25℃
Incubation Time: 30min~30min~15min
Detection limit:
Egg, milk (Method 1) 0.1 ppb
Urine, serum, intestine, feed, milk powder 0.05 ppb
Tissue, liver, honey, milk (Method 2) 0.025 ppb
Recovery rate
Tissue, liver 85±20%
Honey, intestine 83±25%
Milk, feed 75±23%
Urine, serum…………………………………………………………………70±18%
Cross-reaction rate:
Chloramphenicol 100%
Thiamphenicol < 0.1%
Florfeniol < 0.1%

3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3) Enzyme conjugate (12 mL) red cap
4) Concentrated antibody working solution (1 mL) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL) black cap
7) Stop solution (7 mL) yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
9) 2× concentrated redissolving solution (50 mL) transparent cap

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Shenzhen Lvshiyuan Biotechnology Co.,Ltd

Address: D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Longgang, Shenzhen,518120, China, Shenzhen, Guangdong, China, 518120
Tel: 86-755-28438788
Shenzhen Lvshiyuan Biotechnology Co.,Ltd is a new and high technology enterprise, founded by a well-known senior scholars from USA in 2003. We specialize in the most advanced Food Safety ELISA test kit, Animal disease diagnostic kits, small molecule antigen and antibodies, gene recombination antigen and antibodies, etc. and are currently immersed in an ambitious international development process. Dedication and commitment, along with a variety of individual strengths and talents, make each of the employees at Lvshiyuan a real asset to the organization. Our scientific staff has experience with medical, industrial and environment diagnostics. We are able to customize programs to serve client鎶?specific needs with competitive pricing and high quality standards. Shenzhen Lvshiyuan Biotechnology Co.,Ltd is one of the largest and most complete manufacturers on biological diagnostic reagents in China. We firstly commercialize Nitrofurazone, Nitrofurantoin metabolites ELISA test kit and other products in the world. Our products are widely used in government labs, enterprises and research institutes. To meet the needs of market, we are developing hundreds of kits. We take 鎻僽ilding up Green world, Spring from you and our cooperation?as the goal insist that 鎻僫otechnology serves humanity?as the management ideal, provide the leading biological technology products and the perfect technical service for the customers.

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