Carboxypeptidase B Enzymatic synthesis of Specific compounds , producing recombinant Polypeptide - RCPB01

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Carboxypeptidase B  Enzymatic synthesis of Specific compounds , producing recombinant Polypeptide

Carboxypeptidase B ?Enzymatic synthesis of Specific compounds, producing recombinant Polypeptide

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RCPB / rCPB /? Recombinant Carboxypeptidase B / lyophilized powder

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DESCRIPTION

Staphylococcus Aureus v8 Protease

Carboxypeptidase?B?catalyzes?hydrolysis?of?the?basic?amino?acids?lysine,?arginine?and?histidine?from?the

?C-terminal?end?of?polypeptides.?The?molecular?weight?is?34,500?daltons,?the?pH?optimum?is?8.0,?and?pI?is?

6.0.Carboxypeptidase?B?is?competitively?inhibited?by?arginine?and?lysine.?The?enzyme?is?also?inhibited?by?

metalchelating?agents,?e.g.,?EDTA.?Recombinant?Carboxypeptidase?B?(EC?3.4.17.2)?is?expressed?in?E.Coli?

and purified?by?high?pressure?liquid?chromatography.?There?is?no?trace?of?other?enzyme?(such?as?

carboxypeptidase

?A?and?chymotrypsin)?activity.?No?protease?inhibitors?such?as?PMSF?are?present?in?the?preparation.

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MAIN?FEATURES

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1. Source????????????????????? E.?Coli

2. Purified?by???????????????? HPLC?

3. Physical?form???????????? White?or?white-like?lyophilized?powder

4. Additives??????????????????? Tris?salts,?NaCl?salts?and?carbohydrates

5. Protein?content????????? 35%?~?50%

6. Specific?activity?????????? ≥120?units/mg?pro.;?≥170?units/mg?pro

7. Purity????????????????????????? >?95%

8.Contaminant?activity??? No?chymotrypsin,carboxypeptidase?A,and?other?protease?contaminant.Less?than?10

ppm?of?recombinant?trypsin.

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Shanghai Yaxin Biotechnology Co.,Ltd.

Address: No.466 YinDu Road,XuHui, Shanghai, China, Shanghai, Shanghai, China, 200000
Tel: 86-021-54336592
Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin. Animal original free enzymes are specially used in the human recombinant insulin production, and exporting abroad. Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine Usage of recombinant trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production. YaxinBio insists on 鈥渟cientific and technological innovation鈥?has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep. 2013.

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