Chemical Reagent

Product Description
REAGEN? BTS Combo Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect beta-lactam, tetracycline, and sulfonamide antibiotic residues in raw commingled cow’s milk.This test uses a uses novel antibiotic binding proteins which are highly active in cold milk and eliminate the need for a heating block. This test is designed for rapid field use or reference lab settings. The unique features of the kit are:
? ?Rapid strip test method –7 minutes
? ?Works in cold milk
? ?Novel binding proteins that requires no heating step
? ?High sensitivity
? ?High reproducibility
Procedure Overview
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the antibiotic binding proteins on the gold particles to engage with any antibiotic present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to their respective test line, forming a signal (red line) at that position. Each test strip is imprinted with three test lines and one Control line (C-line)at the top. The top test line specifically detects for beta-lactams