Bio-technology Products

Avian Infectious Bursal Disease virus VP2 antibody
(IBDV-VP2 Ab) ELISA kit
Catalog No. LSY-30014
1. Introduction
The GreenSpring? Avian Infectious Bursal Disease virus VP2 antibody (IBDV-VP2 Ab) ELISA kit is developed to detect the IBDV-VP2 antibodies level in chicken serum sample and can be used to evaluate Infectious Bursal Disease vaccine status in chickens.

2. Principle
This kit is based on solid-phase enzyme-linked immunosorbent assay (ELISA) principle, composed by the reaction Micro-plate coated with high purity IBDV-VP2 antigen, horseradish peroxidase-labeled anti-chicken IgG and other reagents. The reaction mechanism is the coated antigen binding with IBDV-VP2-Ab in sample, and then with the enzyme-labeled anti-chicken IgG antibody to form a & quot;coated antigen + IBDV-VP2-Ab + anti-chicken IgG HRP antibody& quot; complex, add substrate, it will have coloration by the enzyme catalytic reaction. Color depth is proportional to the amount of IBDV-VP2-Ab, when the sample chromogenic reaction, the results detected by the microplate reader exceeds a set threshold value result judged as positive, indicating that the immune produced antibodies or natural infection exists.

3. The kit components
1 IBDV-VP2 antigen coated microplate 96T X2
2 Enzyme conjugate 25 ml Yellow lid
3 Sample diluent 50 ml transparent lid
4 IBDV-VP2-IgGNegative control serum 1.5 ml green lid
5 IBDV-VP2-IgG Positive control serum 1.5 ml red lid
6 Substrate A 12 ml purple lid
7 Substrate B 12 ml orange lid
8 Stop solution 12 ml blue lid
9 20×concentrated washing buffer 50 ml white lid
10 Adhesive Foil 6 pieces
11 Instruction 1 piece

4. Materials Required But Not Provided
1Microplate Reader (double-wave length: 450/630nm).
2Micropipettes: single-channel 1~100l,0.5~10l, multi-channel 30~300l
3Microplate Washer.
4Water bath or incubator.
5Oscillator.
6Disposable tips (10ul, 200ul)
7Deionized water