Biochemical

Product Description

REAGEN? Ascorbic Acid Assay Kit is a cuvette-based enzymatic assay for the determination of Ascorbic Acid across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ascorbic Acid directly from food samples.
The unique features of the kit are:

? High sensitivity and low detection limit (0.6 ppm)
? High reproducibility
? A rapid (25 minute), robust enzyme-based assay.

Procedure Overview

REAGEN? Ascorbic Acid Assay Kit uses a specific visible, colorimetric reaction to detect ascorbic acid in food samples: ascorbic acid first reduces an electron carrier (PMS). This compound then reduces MTT to produce a colored MTT formazan product. This conversion, measured at 578 nm, is proportional to the level of ascorbic acids in the sample.

The absorbance of each sample at 578 nm is measured using a spectrophotometer in a cuvette (or plate reader). The concentration of ascorbic acid in each sample is then directly determined from the change in absorbance at 578 nm within 30 minutes time.