Animal Components Free Carboxypeptidase B For Protein Structure And Sequence Analysis - SRCPB01
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Animal Components Free Carboxypeptidase B For? Protein Structure And Sequence Analysis
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Description
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Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
CAS:9025-24-5
EC:3.4.17.2
Source:Rat carboxypeptidase B, expressed in E. Coli.
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Main Features
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Purity by HPLC | ≥?95% |
Format | Lyophilized |
Specific activity | ≥?200 units/mg pro |
Contaminant activity | No chymotrypsin, carboxypeptidase A, or other proteases contaminant. |
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Application
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Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
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Recommend?Usage
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Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH?7-9.
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Storage Instructions
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Recommend recombinant carboxypeptidase B lyophilized?should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
Shanghai Yaxin Biotechnology Company
Address: No.466 YinDu Road,XuHui, Shanghai, China, Shanghai, Shanghai, China, 20000
Tel: 86-21-54336592
Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise
that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only
a company on researching and producing the recombinant carboxypeptidase B and recombinant
trypsin.
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