Bio-technology Products
Description
Porcine Circovirus (PCV2) ELISA test Kit
Catalog No. LSY-30007
1. Usage
The GreenSpring? porcine circovirous ELISA Test kit is used for detection of porcine circovirus antibodies in the serum; assessment of immunity conditions against porcine circovirus in the pig farm and investigation of the epidemiology of the porcine circovirus.
2. Principle
The GreenSpring? PCV2 ELISA kit is based on an indirect enzymatic immunoassay(Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by ELISA Reader.
3. The kit components
1 PCV Antigen coated microtiter plate 2 pieces (96 wells)
2 Negative (green lid)/positive (red lid)serum 1 tube each (1.5 mL/tube)
3 Enzyme conjugate (yellow lid) 1 bottle (22 mL/bottle)
4 20×concentrated washing buffer (white lid) 1 bottle (50 mL/bottle)
5 Substrate (orange lid) 2 bottles (12 mL/bottle)
6 Stop solution(blue lid) 1 bottle (12 mL/bottle)
7 Sample diluent solution(transparent lid) 1 bottle (50 mL/bottle)
8 Adhesive Foil 6 pieces
9 Instruction 1 piece
4. Materials Required But Not Provided
1Microplate Reader (double-wave length: 450/630 nm).
2Precise micropipette (single-channel 1-100ul、0.5-10ul、multi-channel 30-300ul)
3Constant temperature box or water bath box.
4Oscillator.
5Microplate Washer.
6Disposable tips (10ul, 200ul)
7 Deionized water
5. Sample requirement
1The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.
2Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
3The EDTA, heparin sodiun and other anticoagulants will not affect the results.
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Shenzhen Lvshiyuan Biotechnology Co.,Ltd
food safety elisa test kit
Address: D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Longgang, Shenzhen,518120, China,
Shenzhen, Guangdong
China, 518120
Tel: 86-755-28438788
Fax: 86-755-28938800
